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Gireesh Kumar, R.
- Isolation and Screening for Efficiency of Organic Phosphorus Pesticide (Chlorpyriphos) Degrading Bacteria from Different Crops
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1 Department of Agricultural Microbiology and Bioenergy, College of Agriculture, Prof. Jaya Shankar Telangana State Agricultural University, Rajendranagar, Hyderabad - 500 030, Telangana, IN
1 Department of Agricultural Microbiology and Bioenergy, College of Agriculture, Prof. Jaya Shankar Telangana State Agricultural University, Rajendranagar, Hyderabad - 500 030, Telangana, IN
Source
Nature Environment and Pollution Technology, Vol 16, No 1 (2017), Pagination: 169-173Abstract
In the present study total eight bacterial isolates were isolated from vegetable crops like tomato, brinjal by the enriched MSM broth with a supplement of chlorpyrifos source. These isolates were characterized morphologically, cultural and biochemically. Isolates of the microbial strains for identifying those having high chlorpyrifos degradation capabilities in liquid culture were undertaken as well. Among the eight chlorpyrifos bacterial isolates, CDB-1 showed the high population count at different incubation periods (1st day to 5th day) compared to all CDB inoculates. The CDB-1 isolate utilized the pesticide (chlorpyrifos) effectively and showed maximum growth bacterial count 5.8 cfu ×106 mL-1. Efficient CDB-1 isolate and chlorpyrifos degrading capacity were determined with measurement of chlorpyrifos residual concentrations at different days of intervals using gas chromatographic method. The degradation of chlorpyrifos at different concentrations (20, 30 and 40 mgL-1) was examined in the MSL medium. By this degradation percentage study of chlorpyrifos revealed that the chlorpyrifos degrading bacterial isolate (CDB-1) degrade the chlorpyrifos effectively. These isolates used the OP compounds as a carbon source.Keywords
Chlorpyrifos Degradation, Minimal Salt Medium, Biochemical Characterization, Pesticide Degradation Ability.References
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